human asyn elisa kit  (BlueGene Biotech)

Journal: NPJ Parkinson's Disease

Article Title: Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

doi: 10.1038/s41531-022-00357-0

Figure Lengend Snippet: Expression of aSyn has been detected in brain tissue, in peripheral tissues as well as in biological fluids. Specifically, in body fluids (blood and CSF), aSyn is circulated both as a free protein and/or exosomes-inbound protein. Created with BioRender.com.

Article Snippet: , Serum , Modified paired surface plasma wave biosensor coupled to an immunoassay and non-labeled technique , Rabbit monoclonal anti-α-syn (phosphor S129) antibody (anti-p-S129-α-syn; Abcam, Cambridge, MA, USA) , 0.5–5 ng/ml , , 4–12 ng/ml , , , , Human phosphorylated aSyn ELISA kit (MyBioSource Co., Vancouver, Canada) , NA , NA , Chen et al. .

Techniques: Expressing

Journal: NPJ Parkinson's Disease

Article Title: Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

doi: 10.1038/s41531-022-00357-0

Figure Lengend Snippet: a A schematic depiction of aSyn PTMs detected in the brains of patients with synucleinopathies by MS studies and immunohistochemistry. b Comparison of the truncated aSyn species identified in the brain and appendix identified by MS and antibody-based approaches. c Overview of aSyn PTMs identified in the peripheral tissues, brain and body fluids. Various aSyn PTMs have been reported to be present in the human brain using different approaches, i.e., MS or antibody-based assays. Remarkably, some of these PTMs have also been identified in different peripheral tissues and body fluids. pS129 aSyn is the most extensively studied disease-related PTM and is reported to be present in various biological specimens. d PTMs of human aSyn in body fluids and assays used to profile, identify and quantify unmodified and modified aSyn species. Schematic depiction of the aSyn PTMs identified in different constituents of the blood [plasma, serum and red blood cells (RBCs)] and CSF.

Article Snippet: , Serum , Modified paired surface plasma wave biosensor coupled to an immunoassay and non-labeled technique , Rabbit monoclonal anti-α-syn (phosphor S129) antibody (anti-p-S129-α-syn; Abcam, Cambridge, MA, USA) , 0.5–5 ng/ml , , 4–12 ng/ml , , , , Human phosphorylated aSyn ELISA kit (MyBioSource Co., Vancouver, Canada) , NA , NA , Chen et al. .

Techniques: Immunohistochemistry, Comparison, Modification, Clinical Proteomics

Journal: NPJ Parkinson's Disease

Article Title: Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

doi: 10.1038/s41531-022-00357-0

Figure Lengend Snippet: Analysis of aSyn PTMs species in the different biological fluid specimens: overview of the different techniques, antibodies employed and aSyn PTMs concentration range across control and patient groups.

Article Snippet: , Serum , Modified paired surface plasma wave biosensor coupled to an immunoassay and non-labeled technique , Rabbit monoclonal anti-α-syn (phosphor S129) antibody (anti-p-S129-α-syn; Abcam, Cambridge, MA, USA) , 0.5–5 ng/ml , , 4–12 ng/ml , , , , Human phosphorylated aSyn ELISA kit (MyBioSource Co., Vancouver, Canada) , NA , NA , Chen et al. .

Techniques: Concentration Assay, Control, Modification, Western Blot, Dot Blot, Enzyme-linked Immunosorbent Assay, Mass Spectrometry, Luminex, Recombinant, Incubation, Phospho-proteomics, Mutagenesis, Clinical Proteomics, Immunodepletion, Electrochemiluminescence, Ubiquitin Proteomics

Journal: NPJ Parkinson's Disease

Article Title: Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

doi: 10.1038/s41531-022-00357-0

Figure Lengend Snippet: a Comparison of CSF levels of total aSyn between PD and controls in different studies. b Sample size of PD and controls used in the studies shown in a. c Variability of CSF levels of total aSyn across other disease-group patients. Each dot in the graph displays the average values of aSyn reported in the respective study. CBD corticobasal degeneration, MCI mild cognitive impairment, VaP vascular parkinsonism, CJD Creutzfeldt–Jakob disease, NPH normal-pressure hydrocephalus, FD frontotemporal dementia.

Article Snippet: , Serum , Modified paired surface plasma wave biosensor coupled to an immunoassay and non-labeled technique , Rabbit monoclonal anti-α-syn (phosphor S129) antibody (anti-p-S129-α-syn; Abcam, Cambridge, MA, USA) , 0.5–5 ng/ml , , 4–12 ng/ml , , , , Human phosphorylated aSyn ELISA kit (MyBioSource Co., Vancouver, Canada) , NA , NA , Chen et al. .

Techniques: Comparison

Journal: NPJ Parkinson's Disease

Article Title: Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

doi: 10.1038/s41531-022-00357-0

Figure Lengend Snippet: Overview of the concentration of unmodified aSyn and pS129 aSyn in the different biological fluid specimens.

Article Snippet: , Serum , Modified paired surface plasma wave biosensor coupled to an immunoassay and non-labeled technique , Rabbit monoclonal anti-α-syn (phosphor S129) antibody (anti-p-S129-α-syn; Abcam, Cambridge, MA, USA) , 0.5–5 ng/ml , , 4–12 ng/ml , , , , Human phosphorylated aSyn ELISA kit (MyBioSource Co., Vancouver, Canada) , NA , NA , Chen et al. .

Techniques: Concentration Assay, Clinical Proteomics, Ubiquitin Proteomics

Journal: NPJ Parkinson's Disease

Article Title: Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

doi: 10.1038/s41531-022-00357-0

Figure Lengend Snippet: a Number of studies that identified CSF aSyn PTMs. b Variability of pS129 levels across the different disease groups and controls. Each dot in the graph corresponds to the average values of pS129 aSyn reported in the respective study. Other aSyn PTMs, i.e., c Levels of pY39 and d oligo-phosphorylation reported in single studies. The legend/color code in a corresponds also to b , c and d .

Article Snippet: , Serum , Modified paired surface plasma wave biosensor coupled to an immunoassay and non-labeled technique , Rabbit monoclonal anti-α-syn (phosphor S129) antibody (anti-p-S129-α-syn; Abcam, Cambridge, MA, USA) , 0.5–5 ng/ml , , 4–12 ng/ml , , , , Human phosphorylated aSyn ELISA kit (MyBioSource Co., Vancouver, Canada) , NA , NA , Chen et al. .

Techniques: Phospho-proteomics

Journal: NPJ Parkinson's Disease

Article Title: Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

doi: 10.1038/s41531-022-00357-0

Figure Lengend Snippet: a Numbers of studies that reported the detection of aSyn PTMs in plasma/serum. b Variability of pS129 concentration between HC and PD in the different studies. Each dot represents the mean concentration of pS129 aSyn reported in each study. The mean of HC and PD is displayed for Cariulo et al. because only one pS129 level was provided in that study. Other aSyn PTMs, namely, c serum 3-nitrotyrosine and d plasma oligo-phosphorylation , were also reported, but only in a single study. Ubiquitinated aSyn levels are not shown because this modification was assessed and quantified only by WB. The legend/color code in a corresponds also to b , c and d .

Article Snippet: , Serum , Modified paired surface plasma wave biosensor coupled to an immunoassay and non-labeled technique , Rabbit monoclonal anti-α-syn (phosphor S129) antibody (anti-p-S129-α-syn; Abcam, Cambridge, MA, USA) , 0.5–5 ng/ml , , 4–12 ng/ml , , , , Human phosphorylated aSyn ELISA kit (MyBioSource Co., Vancouver, Canada) , NA , NA , Chen et al. .

Techniques: Clinical Proteomics, Concentration Assay, Phospho-proteomics, Modification

Journal: NPJ Parkinson's Disease

Article Title: Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

doi: 10.1038/s41531-022-00357-0

Figure Lengend Snippet: a Number of studies that reported the identification of different aSyn PTMs in RBCs. b Variability of pS129 levels across the different studies comparing PD to HC or to patients with other synucleinopathies such as MSA. In this graph, it is also depicted that RBC pS129 levels differ between the membrane and cytosol fractions . Each dot represents the mean levels of pS129 aSyn reported in each study. The levels of aSyn PTMs such as nY39, pY125, AGEs or SUMOylation are not depicted in the figure because they were assessed only by dot and Western blotting analysis ; no assay for obtaining an “absolute” quantification was carried out. The legend/color code in a corresponds also to b .

Article Snippet: , Serum , Modified paired surface plasma wave biosensor coupled to an immunoassay and non-labeled technique , Rabbit monoclonal anti-α-syn (phosphor S129) antibody (anti-p-S129-α-syn; Abcam, Cambridge, MA, USA) , 0.5–5 ng/ml , , 4–12 ng/ml , , , , Human phosphorylated aSyn ELISA kit (MyBioSource Co., Vancouver, Canada) , NA , NA , Chen et al. .

Techniques: Membrane, Western Blot, Quantitative Proteomics

Journal: NPJ Parkinson's Disease

Article Title: Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

doi: 10.1038/s41531-022-00357-0

Figure Lengend Snippet: Male vs. female representation in studies that aimed to measure the levels of a total aSyn in CSF and aSyn PTMs in b CSF, c plasma/serum and d RBCs.

Article Snippet: , Serum , Modified paired surface plasma wave biosensor coupled to an immunoassay and non-labeled technique , Rabbit monoclonal anti-α-syn (phosphor S129) antibody (anti-p-S129-α-syn; Abcam, Cambridge, MA, USA) , 0.5–5 ng/ml , , 4–12 ng/ml , , , , Human phosphorylated aSyn ELISA kit (MyBioSource Co., Vancouver, Canada) , NA , NA , Chen et al. .

Techniques: Clinical Proteomics

Journal: NPJ Parkinson's Disease

Article Title: Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

doi: 10.1038/s41531-022-00357-0

Figure Lengend Snippet: a The combination of (1) amplification and detection of minute amounts of aggregated aSyn in biological samples (i.e., aSyn SAA) coupled with cryo-EM and (2) identification and quantification of aSyn species by MS/MS can lead to the discovery and validation of novel biomarkers, relying on structure-based classification and disease-specific aSyn PTMs. Together, these approaches can open new avenues to enable differentiating PD patients from controls and from patients with other synucleinopathies. b Schematic depictions illustrating how attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and surface-enhanced infrared absorption spectroscopy (SEIRA) could be used as complementary approaches in this workflow for high-throughput structural analysis of biological samples, allowing molecular-level differentiation of a monolayer of aSyn monomers and fibrils. Created with BioRender.com and the cryo-EM structures of the aSyn fibrils depicted in the figure are derived from different aSyn recombinant proteins.

Article Snippet: , Serum , Modified paired surface plasma wave biosensor coupled to an immunoassay and non-labeled technique , Rabbit monoclonal anti-α-syn (phosphor S129) antibody (anti-p-S129-α-syn; Abcam, Cambridge, MA, USA) , 0.5–5 ng/ml , , 4–12 ng/ml , , , , Human phosphorylated aSyn ELISA kit (MyBioSource Co., Vancouver, Canada) , NA , NA , Chen et al. .

Techniques: Amplification, Cryo-EM Sample Prep, Tandem Mass Spectroscopy, Biomarker Discovery, Fourier Transform Infrared Spectroscopy, Spectroscopy, High Throughput Screening Assay, Derivative Assay, Recombinant

Journal: Antioxidants

Article Title: Native α-Synuclein, 3-Nitrotyrosine Proteins, and Patterns of Nitro-α-Synuclein-Immunoreactive Inclusions in Saliva and Submandibulary Gland in Parkinson’s Disease

doi: 10.3390/antiox10050715

Figure Lengend Snippet: Individual total αSyn concentration (pg/mL) in ( A ) saliva and ( B ) serum in patients with IPD and control participants, as measured with ELISA. Mean and standard deviation are represented with solid lines. Abbrev.: αSyn, α-synuclein; IPD, idiopathic Parkinson’s disease; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: The α-Synuclein concentration was evaluated with a commercially available Enzyme-linked Immunosorbent Assay kit (Human aSyn ELISA Kit, cat. #E09S0131, Shanghai BlueGene Biotech CO., LTD, Shanghai, China), following manufacturer’s instructions.

Techniques: Concentration Assay, Control, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Antioxidants

Article Title: Native α-Synuclein, 3-Nitrotyrosine Proteins, and Patterns of Nitro-α-Synuclein-Immunoreactive Inclusions in Saliva and Submandibulary Gland in Parkinson’s Disease

doi: 10.3390/antiox10050715

Figure Lengend Snippet: Individual 3-NT-proteins concentration (µg/mL) in ( A ) saliva and ( B ) serum in patients with IPD and control participants, as measured with ELISA. Mean and standard deviation are represented with solid lines. Abbrev.: 3-NT-proteins, 3-nitrotyrosine proteins; IPD, idiopathic Parkinson’s disease; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: The α-Synuclein concentration was evaluated with a commercially available Enzyme-linked Immunosorbent Assay kit (Human aSyn ELISA Kit, cat. #E09S0131, Shanghai BlueGene Biotech CO., LTD, Shanghai, China), following manufacturer’s instructions.

Techniques: Concentration Assay, Control, Enzyme-linked Immunosorbent Assay, Standard Deviation